Hi Gloria,
Thanks for looking into this. Yes, the issue is still very much here. I've actually re-imaged my machine yesterday only to experience the same issue in the new system.
To answer your questions:
- See my initial post for version ID. After re-imaging, I am using Office 16.0.10228.20080 32-bit - in which I continue to experience the same issue.
- It depends:
- when opening multiple files at once (i.e. 11 files) 4-5 files will open in the first instance, before new instance gets launched for the remaining files.
- for opening xlsx Outlook attachments, it can be as early as the first file (i.e. Excel running with one file open, double-clicking Outlook attachment launches new instance).
Additionally, I recorded the problem related to opening xlsx attachments from Outlook in a gif file below.
I start from un-hiding my personal macro workbook, so that you can see there are no other files open. Then I attempt to open Outlook attachment by double-clicking on it. It looks like it takes Excel a few seconds to react - perhaps this is useful in identifying
the cause of the issue. And as you can see, it launches the file in new instance. See more comments below.

Regarding your other comments:
Safe mode - not sure why this was suggested, none of my Office apps want to launch while Windows is in safe mode (see below).

XLSTART folde r - I'm sorry if that wasn't clear in my initial post. Let me explain it better. This isn't about opening multiple files from XLSTART folder. It's about opening multiple files (from any folder) when in my first
and only Excel instance a file that sits in XLSTART folder is currently open (i.e. my personal macro workbook). In this example, the moment the 2nd instance launches and generates the "File in use" dialog box, every remaining file will launch a new instance
for itself, each generating the same dialog box.
Example:
My Excel is running (one instance only) and my personal macro workbook (located in XLSTART folder) is open in it. Now, I want o open 11 .xlsx files at once. I go to the folder with my files (i.e. C:\Users\Me\Documents), select all 11 files, right-click one
and press Open. My files begin to open. Files 1-4 all open in my first Excel instance, but, for some reason, file #5 launches a new instance. The new instance tries to open my personal macro workbook in XLSTART folder, but it is already open in my first instance.
So the "File in use" warning message pops-up in the 2nd instance and awaits my input/selection. Meanwhile, file #6 wants to open. I can only assume that it tries to use the 2nd instance, but because the 2nd instance is awaiting my input, it launches a 3rd
instance. The third instance attempts to open my personal macro workbook in XLSTART folder and generates the same warning message. Then file #7 wants to open and it goes through the same cycle of launching new instance because the last one awaits user input
and triggering the below warning message. At the end of this loop, I have
8 Excel instances open, 1st one has my personal macro workbook and files 1-4 open, and instances 2nd through 8th are displaying "File in Use" warning message.

Hope this is all clear now, let me know if not.
Thanks,
J.