Hello Albert Cooper,
Based on the error, it sounds like the file is being processed through the chat attachment pipeline rather than being accessed as a knowledge source. The FileValidationError and the reported 16 MB limit suggest the file is failing validation before the agent can review it, even though other Copilot scenarios may support larger file sizes. Could you confirm the file type (PDF, DOCX, PPTX, etc.), its actual size, and whether the issue occurs with all files or only specific ones?
It would also be helpful to know whether the file is being uploaded directly in chat, accessed through a SharePoint link, or retrieved from a SharePoint library configured as a knowledge source. If the same error occurs when using a SharePoint link, the service may still be attempting to ingest the document through the attachment-processing path rather than retrieving it from SharePoint. If this previously worked and no configuration changes were made, it could indicate a recent platform regression. Capturing a correlation ID, conversation ID, sample file details, and whether the issue reproduces in both the test and published experiences would help determine for further investigation.